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Mycobacterium tuberculosis 1

Thermus thermophilus 1

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ion-preference 1

methionine adenosyltransferase 1

secondary structure 1

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Functional characterization of a thermostable methionine adenosyltransferase from

Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 2,   Pages 238-244 doi: 10.1007/s11705-016-1566-2

Abstract: MATTt (a thermostable methionine adenosyltransferase from HB27) was overexpressed in and purified using Ni-NTA affinity column. The enzymatic activity of MATTt was investigated in a temperature range from 30 °C to 90 °C, showing that MATTt exhibited a high enzymatic activity and good thermostability at 80 °C. Circular dichroism spectra reveals that MATTt contains high portion of -sheet structures contributing to the thermostability of MATTt. The kinetic parameter, is 4.19 mmol/L and 1.2 mmol/L for ATP and methionine, respectively. MATTt exhibits the highest enzymatic activity at pH 8. Cobalt (Co ) and zinc ion (Zn ) enhances remarkably the activity of MATTt compared to the magnesium ion (Mg ). All these results indicated that the thermostable MATTt has great potential for industry applications.

Keywords： ion-preference     methionine adenosyltransferase     secondary structure     thermostability     Thermus thermophilus    

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PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing Article

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Engineering 2022, Volume 15, Issue 8,   Pages 67-77 doi: 10.1016/j.eng.2022.02.013

Abstract: By screening distinct CRISPR base editors, we discovered that only the unusual Streptococcus thermophilus

Keywords： CRISPR     Cas9     Mycobacterium tuberculosis     Genome editing     Base editing    

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Title Author Date Type Operation

Functional characterization of a thermostable methionine adenosyltransferase from

Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan

Journal Article

PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Journal Article